ONKI3 | Medical Subject Headings

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Polymerase Chain Reaction

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Hierarchy:	Medical Subject Headings (MeSH) ∟ Investigative Techniques ∟ Genetic Techniques ∟ Nucleic Acid Amplification Techniques ∟ Polymerase Chain Reaction 2 coordinate concepts 3 subordinate concepts ∟ Primed In Situ Labeling \| ∟ Random Amplified Polymorphic DNA Technique \| ∟ Reverse Transcriptase Polymerase Chain Reaction ∟ Ligase Chain Reaction \| ∟ Self-Sustained Sequence Replication X
Broader terms:	Medical Subject Headings (MeSH) ∟ Investigative Techniques ∟ Genetic Techniques ∟ Nucleic Acid Amplification Techniques ∟ Polymerase Chain Reaction Nucleic Acid Amplification Techniques > Genetic Techniques > Investigative Techniques > Medical Subject Headings (MeSH) X
Narrower terms:	Primed In Situ Labeling \| Random Amplified Polymorphic DNA Technique \| Reverse Transcriptase Polymerase Chain Reaction X
History note:	Gene Amplification (1988-1989)X
historyNote*:	91(90); was see under GENE AMPLIFICATION 1990 X
publicMeSHNote*:	91; was see under GENE AMPLIFICATION 1990 X
Scope note:	In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships. X
activeMeSHYear*:	2007X
dateCreated*:	1990-01-11X
dateEstablished*:	1991-01-01X
dateRevised*:	2006-07-05X
recordAuthorizer*:	sjnX
recordMaintainer*:	agsX
recordOriginator*:	PLSX
Related term:	Ligase Chain Reaction \| Sequence Tagged Sites X
Type:	Concept X
Coordinate terms:	Ligase Chain Reaction \| Self-Sustained Sequence Replication X
URI:	X
Labels and equivalent concepts:	Polymeraasiketjureaktio (fi) PCR (fi, replaced) Polymeraskedjereaktion (sv) PCR (sv, replaced) PCR (en, replaced) Nested PCR (en, replaced) Inverse Polymerase Chain Reaction (en, replaced) Anchored Polymerase Chain Reaction (en, replaced) X
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